macrovipera lebetina venom

Abstract: The activity of phospholipase A2 in the venom of the Transcaucasian Macrovipera lebetina obtusa, collected from viperas inhabiting in different regions of Azerbaijan in terms of pollution, was studied.The lowest (30,2 IU / mg) was detected in … ... Snake venom vascular endothelial growth factor toxin ICPP 1 Publication Macrovipera lebetina obtusa (Dwigubsky, 1832) and Montivipera xanthina (Gray, 1849) (Ottoman Viper) are viper snakes from Viperidae family and found in various locations in Anatolia. Common names: blunt-nosed viper, Lebetine viper, Levant viper, more. WCH Clinical Toxinology Resources Macrovipera lebetina obtusa (MLO) venom was pooled from 20 adult individuals between 90 and 120cm in length and older than 3 years of both sexes, collected during the day in spring and autumn in different regions of the Republic of Armenia. Key words: snakebite, snake envenomation, antivenom, anti-snake venom, Levantine viper, snakes of medical significance, developing world, Macrovipera lebetina Until recently, the entirely unhelpful title of the ‘‘Big Four’’ snakes of medical significance has predominated in India. Dynamic Changes in Lipid Peroxidation and Antioxidant ... Both snakes are responsible for major snake bite cases in Turkey Their venoms cause necrosis, hemorrhage, pain and local edema. Sameh Sarray 1,5, … Despite the viper's large size, data on its body mass and sex-specific morphological differences are scarce. The presence of viper venom in organism lead to increasing of the electrical resistance of BLMs from liver and muscle … It is a monomeric protein with a molecular mass of 25,787 Da. The aim of the present study was to determine the in vitrocytotoxicity of Macrovipera lebetina obtusa(Blunt-Nosed Viper) crude venom from southeastern Anatolia against K562 human chronic myelogenous leukemia (CML) cells by 3-(4,5-dimethylthiazol-2-yl)-2,5-di- phenyltetrazolium bromide (MTT) and Adenosine tripohsphate (ATP) assays. The venoms of Tunisian wildlife snakes are complex mixtures containing proteins/ peptides and non-protein molecules. This snake is found in India in the mountainous regions of Jammu and Kashmir and is capable of causing necrosis and hemostatic manifestations, as seen in the case reported here. Read more related scholarly scientific articles and abstracts. The objective of this study was to investigate the in vitro effect of Vipera lebetina venom on human erythrocytes' osmotic fragility with respect to different venom concentrations, time of incubation with the venom, initial volume of red blood cells, and presence of antivenom. (2004). Export Figures View in gallery Levant blunt-nosed viper Macrovipera lebetina obtusa. We investigated the balance of free radicals in different tissues (liver, heart, brain and muscle) of rats in course of in vivo and in vitro processing by Macrovipera lebetina obtusa (MLO) and Montivipera raddei (MR) snake venoms. Snake venom vascular endothelial growth factor toxin ICPP ... by Abhinandan Chowdhury, Christina N Zdenek, James S Dobson, Lachlan A Bourke, Raul Soria, Bryan G Fry. Lebein, is an heterodimeric disintegrin isolated from Macrovipera lebetina snake venom that was previously characterized as an inhibitor of ADP‐induced platelet aggregation. (C) Map showing the distribution of the five cases of M. l. obtusa envenomation in four provinces of Iran discussed here. Venomics, antivenomics, and neutralization assays of the lethal and toxic venom activities by anti-Macrovipera lebetina turanica and anti-Vipera berus berus antivenoms. Dagestan blunt-nosed viper, Macrovipera lebetina obtusa ... @article{article, year = {2014}, pages = {37-37}, keywords = {European viper venom antiserum, Zagreb antivenom, Montivipera raddei, Macrovipera lebetina obtusa, Paraspecificity, Snake venom, Snake venom metalloproteinases, Snake venom serine proteinases}, title = {Paraspecificity of Vipera a. ammodytes-specific antivenom towards Montivipera raddei and … The venom is then diluted and injected into a … Effect of quercetin's antihemorrhagic activity against Macroviperal ebetina obtusa venom was assessed by histochemical analysis and aspartate transaminase (AST), alanine transaminase (ALT), creatinin and coagulation homeostasis parameters of blood plasma of Wistar rats whit intraperitoneal administration of viperian venom, quercetin+venom and saline buffer. Reviewed-Annotation score: -Experimental evidence at protein level i. Thus investigated crystal of snake venom was pasted on a metal substrate by silver paste. The snake is found in the natural hilly habitats of Cyprus and North Africa among other places. (PDF) A preliminary investigation into the venom proteome ... Osmotic fragility of human erythrocytes in vitro using ... It is the only snake in Cyprus which can be dangerous to humans (9). The venom with which the authors worked was a mixture from about a hundred of these snakes captured in Algeria. Phylogeny and systematics of viperine snakes. The radiation background of radionuclides was determined in the snake venom, soil, water and plant samples taken from the habitation area of the Macrovipera lebetina The glands that secrete zootoxins are a modification of the parotid salivary glands found in other vertebrates and are … Its nominate subspecies Macrovipera l. lebetina is confined to Cyprus, where it is the only dangerously venomous snake species and heavily pursued. Ecology factor and Venom of snake Macrovipera lebetina obtusa The genus Macrovipera, a smaller and taxonomically simpler genus than Echis, currently contains just three species and five subspecies . Lebectin, a novel C-type lectin from Macrovipera lebetina venom, inhibits integrinmediated adhesion, migration and invasion of human tumour cells. Vol-2, Issue-5, Sep-Oct- 2017 ISSN: 2456-1878. To investigate this point, we looked at the in vitro effect of M. lebetina crude venom, using cultured human embryonic kidney (HEK-293) mono layers as a model. Viper venom disintegrins as modulators of integrin activity in the processes of tumor cell proliferation and platelet aggregation. Human brain microvascular endothelial cells (HBMEC), used as an in vitro model, express αvβ3, αvβ5, and α5β1 integrins, as well as the α2, α3, α6, and β4 subunits. ... Bites are painful and a great deal of venom can be injected. We investigated the balance of free radicals in different tissues (liver, heart, brain and muscle) of rats in course of in vivo and in vitro processing by Macrovipera lebetina obtusa (MLO) and Montivipera raddei (MR) snake venoms. This is the first report that describes the in vivo and in vitro toxic effects of Macrovipera lebetina lebetina venom and antivenom production against this species. The complete MVL-PLA2 cDNA was cloned from Macrovipera lebetina transmediterranea venom gland cDNA library. Human brain microvascular endothelial cells (HBMEC), used as an in vitro model, express αvβ3, αvβ5, and α5β1 integrins, as well as the α2, α3, α6, and β4 subunits. injected into the vein of a 2 kgm. [7] För en mer … Allon and Kochva (1974) demonstrated that Palestine vipers (Daboia palaestinae) possess extremely large amounts of venom. Integrins α v β 3 and α 4 β 1 have been shown to bind mammalian GIIA sPLA 2, while an acidic GIIA sPLA 2 from venom of the viper Macrovipera lebetina transmediterranea was shown to interact with integrins α 5 β 1, α v β 3 and α v β 6, to induce inflammation and inhibition of cell adhesion and migration and angiogenicity 36, 103. This viper species is a large snake, with females reaching 214 cm in length and males growing to a similar size. The paper presents data on the study of the thermoprotective properties of the venom of the Transcaucasian Vipeta (Macrovipera lebetina obtuse) on 120 white mongrel mice subjected to thermal exposure (30 - 50°C) for 30 minutes with the injection of zootoxin in doses (2 mg / kg and 4 mg / kg). This article reports for the first time the medical significance of the Levantine viper (Macrovipera lebetina) in India. We demonstrate that this new viper venom antiserum is pre-clinically effective in neutralizing lethal toxicity and hemorrhagicity of … of blood, the … Proteomic analysis of Macrovipera lebetina (blunt-nosed viper) venom revealed the presence of two NP-like peptides in its venom, namely, lebetins 1 and 2 [40]. Venom and antivenoms Venom from Macrovipera lebetina obtusa (Mlo) was pooled from 10 adult individuals between 80 and 110 cm in length and older than 3 years, of both sexes, collected, during the day in spring and autumn and in summer nights, in different regions of the Republic of Dagestan (Russian Federation). Venom from Macrovipera lebetina obtusa (Mlo) was pooled from 10 adult individuals between 80 and 110 cm in length and older than 3 years, of both sexes, collected, during the day in spring and autumn and in summer nights, in different regions of the Republic of Dagestan (Russian Federation). Antivenom, also known as antivenin, venom antiserum, and antivenom immunoglobulin, is a specific treatment for envenomation.It is composed of antibodies and used to treat certain venomous bites and stings. 2.1. Methods: The effect of M. lebetina snake venom on HEK-293 growth inhibition was determined by the … It was found that the lifespan of mice was increased with respect to a … doi: 10.1016/0304-4165(91)90156-B. During the first stage, the animals were injected with MLO venom; to decrease the chance of tissue damage with venom and adjuvant, the injections made in two different anatomical regions on the animal’s neck in areas close to axillary lymph nodes. of tissues in the course of viper’s venom processing in vitro (above—Macrovipera lebetina obtuse; below—Montivipera rad- dei; blue bars—control, purple bars—assays after venom proc-essing). This snake is found in India in the mountainous regions of Jammu and Kashmir and is capable of causing necrosis and hemostatic manifestations, as seen in the case reported here. The CV-short and ML-(6,9,10) encoded disintegrins display high amino acid sequence similarity to the KTS-disintegrins obtustatin, from Macrovipera lebetina obtusa venom [20,21], and viperistatin, isolated from the venom of Vipera palestinae (Figure 3). Macrovipera lebetina obtusa venom to the experimental animal was accompanied by the survival of the majority of mice (Tables 1, 2, 3). ABSTRACT. This species contains several sub-species, which are characterized by a very specific combination of components (Trummal Ketal., 2005; Kallech-Ziri We recently reported that lebectin, a novel C-type lectin from Macrovipera lebetina venom, displays an anti-integrin activity. Hybridization of Vipera schweizeri (Werner, 1935) and Vipera lebetina obtusa, Dvigubsky 1832. All Posts. Viperidae is one family of venomous snakes that is found nearly worldwide. Lebectin, a Macrovipera lebetina venom-derived C-type lectin, inhibits angiogenesis both in vitro and in vivo. The genus Macrovipera, a smaller and taxonomically simpler genus than Echis, currently contains just three species and five subspecies . Background: The blunt-nosed viper Macrovipera lebetina (Linnaeus, 1758) is a medically important snake species in the Middle East. In this study, we extend this observation to lebecetin, a second C-type lectin isolated from the same venom and previously reported as a potent inhibitor … It was established that at heating up to the temperature of 1200C, crystals of Macrovipera Lebetina Obtusa venom have maximum resistance, and after cooling its property restores. Our data also argue … Matrix Biol. Thus, the structure for the further research of electrophysical parametres of venom Caucasian Induces capillary permeability and angiogenesis through VEGF receptor (KDR/VEGFR-2) signaling. Read "A preliminary investigation into the venom proteome of Macrovipera lebetina obtusa (Dwigubsky, 1832) from Southeastern Anatolia by MALDI-TOF mass spectrometry and comparison of venom protein profiles with Macrovipera lebetina lebetina (Linnaeus, 1758) from Cyprus by 2D-PAGE, Archives of Toxicology" on DeepDyve, the largest online rental service for … Function i. Amphibia-Reptilia 13: 375-392 8. By Najet Srairi. Macrovipera lebetina obtusa venom’s crystal were carried out. Macrovipera lebetina (Levantine viper) (Vipera lebetina) Status. Chemiluminescence (ChL) levels were examined in tissue assays after incubation (at 37 °C for a period of 10 min) with venom for in vitro experiments and … The present study was undertaken to elucidate how the plastic properties of model membranes from native lipids of different tissues of rats change in the course of Macrovipera lebetina obtuse (MLO), Montivipera raddei (MR) and Naja kaouthia (NK) venoms processing. Immunization included two stages (Table 1). Biochim Biophys Acta. Characterization and inflammation challenge dose of this venom (37.5 μg/paw) was also determined by the same study. Here, we report the purification and characterization of an acidic Asp49 phospholipase A2, named MVL-PLA2, with a molecular mass of 13,626.64 Da. Induces capillary permeability and angiogenesis through VEGF receptor (KDR/VEGFR-2) signaling. The nominate subspecies Macrovipera lebetina lebetina is restricted to Cyprus (and possibly some localities in Southeastern Turkey). Venom was air-dried at room temperature and stored … The determination of snake venom toxins in the blood of patients was carried out using a combination of extraction methods in the blood-0.9% sodium chloride solution, followed by determination on a Hitachi-850 spectrofluorimeter (Japan). Anahtar Sızc kler:Macrovipera lebetina, hemipenis, venom elektroforezi, KÝbrÝs, G ney Anadolu Introduction The Levantine viper, Macrovipera lebetina, has a rather extensive geographical range in Central Asia, the Middle East and northern Africa (Nilson and Andren, The difference in the toxicity can be explained by the bigger size and higher amount of venom injected by Macrovipera lebetina obtusa, but also the … Human brain microvascular endothelial cells (HBMEC), used as an in vitro model, express αvβ3, αvβ5, and α5β1 integrins, as well as the α2, α3, α6, and β4 subunits. In this study, we investigated the effect of Lebein on the p53‐dependent … The Levantine viper, Macrovipera lebetina, is a venomous viper species found, in Cyprus, the Middle East, northern Africa, Cyclades, Turkey and central Asia (4-8). (B) Razi™ polyvalent antivenin, the only available antivenom for the treatment of snakebite envenomation in Iran. The specific antivenom needed depends on the species involved. Reviewed-Annotation score: -Experimental evidence at protein level i. γ – radiospectrometric studies showed that the samples of venom also contain radionuclides as ra228, … Although structurally similar to NPs, lebetins exhibit potent anti-platelet activity and the N-terminal extension of these peptides is identified to be the region responsible for this activity [ 41 ]. Has hydrolytic activities against BAEE (1.2 U/mg), TAME, and Pro-Phe-Arg-MCA (4.9 U/mg). This article reports for the first time the medical significance of the Levantine viper (Macrovipera lebetina) in India. The subspecies lebetina seems to be endemic to Cyprus. Lebecin, a new C-type lectin like protein from Macrovipera lebetina venom with anti-tumor activity against the breast cancer cell line MDA-MB231. abstract: in this article presents experimental data, the basic composition of the venom of macrovipera lebetina obtusa, captured from different regions of azerbaijan, differing in degree of contamination by industrial emissions was studied. The venoms of these vipers have been the subject of many studies and are considered as a promising source of bioactive molecules. of tissues in the course of viper’s venom processing in vitro (above—Macrovipera lebetina obtuse; below—Montivipera rad- dei; blue bars—control, purple bars—assays after venom proc-essing). In vitro osmotic fragility of human erythrocytes was determined using Dacie and Lewis method. It is thus a class II snake of medical significance, as defined by the World Health … Macrovipera. Venom and antivenoms Venom from Macrovipera lebetina obtusa (Mlo) was pooled from 10 adult individuals between 80 and 110 cm in length and older than 3 years, of both sexes, collected, during the day in spring and autumn and in summer nights, in different regions of the Republic of Dagestan (Russian Federation). Snake venom is a highly toxic saliva containing zootoxins that facilitates in the immobilization and digestion of prey.This also provide defense against threats. Thus, electrophysical properties of snake venom were experimentally revealed. Shows coagulant activity. Its nominate subspecies Macrovipera l. lebetina is confined to Cyprus, where it is the only dangerously venomous snake species and heavily pursued. In large specimens, 500 to over 1,000 mg of venom may be contained in their venom glands. Despite the viper’s large size, data on its body mass and sex-specific morphological differences … ANTI-VENOM. Anti-venom is created by milking venom from a relevant snake, spider, insect, or fish. Chemiluminescence (ChL) levels were examined in tissue assays after incubation (at 37 °C for a period of 10 min) with venom for in vitro experiments and … (A) Photo of M. l. obtusa captured by Bahman Zangi. A preliminary investigation into the venom proteome of Macrovipera lebetina obtusa (Dwigubsky, 1832) from Souheastern Anatolia by MALDI-TOF mass spectrometry and comparison of venom protein profiles with Macrovipera lebetina lebetina (Linnaeus, 1758) from Cyprus by 2D-PAGE. The focus of our study is the venom of Macrovipera lebetina obtusa (MLO) living in Armenia and its effect on the cells of myocardium. Dried lyophilized toxin of MLO and MR was dissolved in Tris-HCl buffer (pH 7.4) with a final concentration of 3 × 10−5 The blunt-nosed viper (Macrovipera lebetina) is a snake whose venom is one of the deadliest in the world. Abstract: The activity of phospholipase A2 in the venom of the Transcaucasian Macrovipera lebetina obtusa, collected from viperas inhabiting in different regions of Azerbaijan in terms of pollution, was studied.The lowest (30,2 IU / mg) was detected in … It is thus a class II snake of medical significance, as defined by the World Health … En stor huggorm med kort och brett huvud med långa gifttänder, gulaktig, Lebectin, a novel C-type lectin from Macrovipera lebetina venom, inhibits integrin-mediated adhesion, migration and invasion of human tumour cells. dosages of venom of Macrovipera lebetina obtusa snake. There are currently five subspecies including this nominate snake that belong to the blunt-nose viper. Macrovipera lebetina is the most prominent species from this genus, with an extensive geographic range from the Middle East extending throughout central Asia [64,65]. Archives. Shows coagulant activity. Une morsure de serpent est une blessure occasionnée par un serpent, résultant généralement de la pénétration des crochets de cet animal dans le corps, pouvant s'ensuivre d'une envenimation.Bien que la majorité des espèces de serpents ne soient pas venimeuses et étouffent leurs proies par constriction plutôt qu'en les paralysant avec leur venin, on trouve des espèces … In this study, we investigated the anti‐angiogenic activity of lebectin, a newly discovered CLP from Macrovipera lebetina venom. DOI: 10.22161/ijeab/2.4.69 Keyword: phospholipase A2, venom, snake, Macrovipera lebetina obtusa, heavy metals, enzyme activity. [Google Scholar] Function i. Pilorget, A. et al. Macrovipera lebetina har en vidsträckt utbredning från östra Medelhavsområdet (inklusive Turkiet och västra Nordafrika) samt södra Ryssland österut till Kazakstan och Tadzjikistan, söderut till Afghanistan, Pakistan och nordvästra Indien. Macrovipera. The blunt-nosed viper Macrovipera lebetina (Linnaeus, 1758) is a medically important snake species in the Middle East. This definition has been largely propagated by Russ. Lebecetin, a potent antiplatelet C-type lectin from Macrovipera lebetina venom Abstract A novel C-type lectin protein (CLP), lebecetin, was purified to homogeneity from the venom of Macrovipera lebetina by gel filtration on a Sephadex G75 column and ion … This review provides a summary of the different toxins that have been isolated and characterized from the venoms of Vipera lebetina (Macrovipera lebetina) subspecies Macrovipera lebetina cernovi, Macrovipera lebetina lebetina, Macrovipera lebetina obtusa, Macrovipera lebetina transmediterranea, Macrovipera lebetina turanica, the snake species causing the majority of … JOIN OUR NEWSLETTER Enter your E-mail . It cleaves the Arg(1545)-Ser(1546) linkage in the human factor V molecule. MVL-PLA2 possesses 122 amino acid residues, including 14 cysteines, and belongs to group II snake venom … In this study, we investigated the anti-angiogenic activity of lebectin, a newly discovered CLP from Macrovipera lebetina venom. Macrovipera lebetina (Levantine viper) (Vipera lebetina) Status. ... Snake venom vascular endothelial growth factor toxin ICPP 1 Publication The specific binding of purified antibodies were confirmed by enzyme-linked immunosorbent assay. In this study, we investigated the anti-angiogenic activity of lebectin, a newly discovered CLP from Macrovipera lebetina venom. Venom was vacuum-dried at room temperature and stored at -5 o C until used. Venom and antivenoms. J. However, three main vipers exist in the Middle Eastern region: Montivipera bornmuelleri, Macrovipera lebetina, and Vipera (Daboia) palaestinae. Leberagin-C, a new member of the disintegrin-like/cysteine-rich (D/C) family, was purified to homogeneity from the venom of Tunisian snake Macrovipera lebetina transmediterranea. Antivenin (or anti-venom or anti-venene) is a biological product used in the treatment of venomous bites or stings. It is considered an animal of major medical importance that stays calm and peaceful during the day and becomes highly active at night. DOI: 10.22161/ijeab/2.4.69 Keyword: phospholipase A2, venom, snake, Macrovipera lebetina obtusa, heavy metals, enzyme activity. Turkish Journal of Zoology 30:225-234 36. Macrovipera razii; 3. Siigur E., Siigur J. Purification and characterization of lebetase, a fibrinolytic enzyme from Vipera lebetina (snake) venom. However, sizes vary between different populations. We report the cloning and sequence analysis of Cerastes vipera and Macrovipera lebetina transmediterranea cDNAs coding for short non-RGD (Arg-Gly-Asp) disintegrins and for dimeric disintegrin subunits. Consistent with these clinical effects, Macrovipera lebetina venom has been shown to be procoagulant by activating Factor X and Factor V (Siigur et al., 2001a). Disintegrins have numerous applications in studies on platelet thrombosis, endothelial cell apoptosis, migration and angiogenesis. The Levantine viper (Macrovipera lebetina) or blunt-nosed viper is a large venomous snake of the order Squamata and phylum Chordata. Macrovipera lebetina: General Details, Taxonomy and Biology, Venom, Clinical Effects , Treatment ... Average Venom Qty 75 to 150 mg ( dry weight ), Minton (1974) ( Ref : R000504 ). A preliminary investigation into the venom proteome of Macrovipera lebetina obtusa (Dwigubsky, 1832) from Southeastern Anatolia by MALDI-TOF mass spectrometry and comparison of venom protein profiles with Macrovipera lebetina lebetina (Linnaeus, 1758) from Cyprus by … crystals of Macrovipera Lebetina Obtusa venom doesn’t have photoconductivity. The LD as well as HD concentration of the crude venom were obtained to be 45 μg and 120 μg/ml, respectively. Isolation and characterization of a myotoxin from the venom of Macrovipera lebetina transmediterranea Read More. J Cell Physiol. The other dangerous snake Macrovipera lebetina obtusa (Dwigubsky, 1832) is known from Southeastern, venom, our team reported the significant effect of the Artemisia ab-sinthium extract at 50mg/kg on rats, previously. rabbit proved fatal in 10 hours, 8-10 mgm. III. Lebecetin and lebectin, purified from Macrovipera lebetina venom, are the only snaclecs, until today, with an evident anti-tumor effect in addition to their anti-aggregation activity on platelets. Venom serine protease that converts factor V (F5) to the active form Va in the presence of calcium ions and phospholipids. A preliminary investigation into the venom proteome of Macrovipera lebetina obtusa (Dwigubsky, 1832) from Southeastern Anatolia by MALDI-TOF mass spectrometry and comparison of venom protein profiles with Macrovipera lebetina lebetina (Linnaeus, 1758) from Cyprus by 2D-PAGE more 211 307–315 (2007). Its range includes Cyprus Island (represented by nominate subspecies), … The mRNAs belong to the short-coding class, suggesting that these disintegrin mRNAs may be more widely distributed than previously thought. Morphological, hemipenial and venom electrophoresis comparisons of the Levantine viper, Macrovipera lebetina (Linnaeus, 1758), from Cyprus and Southern Anatolia. |Article| doi: 10.1021/jcp.20935 ; Sarray, S. et al. For instance, Phospholipases type A2 (PLA2s) from the Tunisian vipers Cerastes cerastes and Macrovipera lebetina has been found to have anti-tumor activity. The blunt-nosed viper (Macrovipera lebetina) is composed of seven subspecies which inhabit semi-arid environments in North Africa and in the Near and Middle East (Irano-Turanian region). Anti-cancer activity has been also reported for other compounds in snake venom. Has hydrolytic activities against BAEE (1.2 U/mg), TAME, and Pro-Phe-Arg-MCA (4.9 U/mg). 2.1. Venom serine protease that converts factor V (F5) to the active form Va in the presence of calcium ions and phospholipids. C69 neutralized 48% of the hemolysis activity of M. lebetina venom and C37 survived 66% of mice after 115 min of envenoming. 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